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thp 1 reporter cell line assays thp 1 sting lucia isg cells  (InvivoGen)


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    InvivoGen thp 1 reporter cell line assays thp 1 sting lucia isg cells
    Thp 1 Reporter Cell Line Assays Thp 1 Sting Lucia Isg Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thp 1 reporter cell line assays thp 1 sting lucia isg cells/product/InvivoGen
    Average 94 stars, based on 96 article reviews
    thp 1 reporter cell line assays thp 1 sting lucia isg cells - by Bioz Stars, 2026-02
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    reporter cell line thp 1 lucia isg  (InvivoGen)
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    InvivoGen reporter cell line thp 1 lucia isg
    (A) <t>THP-1</t> Dual cells were infected with VSV-G pseudotyped, single-cycle NL43-GFP (HIV-1-GFP) virus with three-fold serial dilutions (starting concentration: 1 µg/ml p24). Cell death and infection were scored by flow cytometry two days later using live/dead staining and GFP expression and quantified. (B) THP-1 Dual cells were infected with NL43-GFP in the presence or absence of reverse transcriptase and integrase inhibitors (NVP and RLT; 10 µM each). Infection and cell death were scored as in (A). UT: untreated (no drug), NVP: nevirapine, RLT: raltegravir.
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    (A) THP-1 Dual cells were infected with VSV-G pseudotyped, single-cycle NL43-GFP (HIV-1-GFP) virus with three-fold serial dilutions (starting concentration: 1 µg/ml p24). Cell death and infection were scored by flow cytometry two days later using live/dead staining and GFP expression and quantified. (B) THP-1 Dual cells were infected with NL43-GFP in the presence or absence of reverse transcriptase and integrase inhibitors (NVP and RLT; 10 µM each). Infection and cell death were scored as in (A). UT: untreated (no drug), NVP: nevirapine, RLT: raltegravir.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: (A) THP-1 Dual cells were infected with VSV-G pseudotyped, single-cycle NL43-GFP (HIV-1-GFP) virus with three-fold serial dilutions (starting concentration: 1 µg/ml p24). Cell death and infection were scored by flow cytometry two days later using live/dead staining and GFP expression and quantified. (B) THP-1 Dual cells were infected with NL43-GFP in the presence or absence of reverse transcriptase and integrase inhibitors (NVP and RLT; 10 µM each). Infection and cell death were scored as in (A). UT: untreated (no drug), NVP: nevirapine, RLT: raltegravir.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection, Virus, Concentration Assay, Flow Cytometry, Staining, Expressing

    Undifferentiated THP-1 cells from different sources were infected with VSV-G pseudotyped, single-cycle NL43-GFP at three different viral inputs in the presence or absence of reverse transcriptase and integrase inhibitors (NVP and RLT; 10 µM). (A-B) Infection and cell death were scored by flow cytometry using GFP expression and live/dead stain. C) Supernatants from infected cells were assayed on HEK-Blue IFN-α/β SEAP reporter cells.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: Undifferentiated THP-1 cells from different sources were infected with VSV-G pseudotyped, single-cycle NL43-GFP at three different viral inputs in the presence or absence of reverse transcriptase and integrase inhibitors (NVP and RLT; 10 µM). (A-B) Infection and cell death were scored by flow cytometry using GFP expression and live/dead stain. C) Supernatants from infected cells were assayed on HEK-Blue IFN-α/β SEAP reporter cells.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection, Flow Cytometry, Expressing, Staining

    (A-C) THP-1 Dual WT and STING KO cells were infected with NL43-GFP or left uninfected, in the presence or absence of RT and IN inhibitors. Infection (A) and death (B) were quantified by flow cytometry. ISRE induction (C) was quantified by a luciferase assay from the supernatants of infected cells. (D-F) Experiments were performed exactly as in (C-E), but in overnight PMA-differentiated (25 ng/ml; 24h) THP-1 Dual WT and STING KO cells. ISRE: IFN-stimulated response element.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: (A-C) THP-1 Dual WT and STING KO cells were infected with NL43-GFP or left uninfected, in the presence or absence of RT and IN inhibitors. Infection (A) and death (B) were quantified by flow cytometry. ISRE induction (C) was quantified by a luciferase assay from the supernatants of infected cells. (D-F) Experiments were performed exactly as in (C-E), but in overnight PMA-differentiated (25 ng/ml; 24h) THP-1 Dual WT and STING KO cells. ISRE: IFN-stimulated response element.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection, Flow Cytometry, Luciferase

    Undifferentiated THP-1 cells deficient in SAMHD1 were infected with VSV-G pseudotyped HIV-1-GFP at different viral inputs in the presence or absence of NVP or RLT (10 µM). (A) Cell lysates from WT or SAMHD1 KO cells were analyzed by Western blot, probed for SAMHD1 and tubulin. (B-C) Infection and cell death were scored by flow cytometry. (D) Supernatants from infected cells were assayed on HEK-Blue IFN-α/β SEAP reporter cells.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: Undifferentiated THP-1 cells deficient in SAMHD1 were infected with VSV-G pseudotyped HIV-1-GFP at different viral inputs in the presence or absence of NVP or RLT (10 µM). (A) Cell lysates from WT or SAMHD1 KO cells were analyzed by Western blot, probed for SAMHD1 and tubulin. (B-C) Infection and cell death were scored by flow cytometry. (D) Supernatants from infected cells were assayed on HEK-Blue IFN-α/β SEAP reporter cells.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection, Western Blot, Flow Cytometry

    (A) THP-1 WT cells were infected with NL43-GFP or not, in the presence or absence of NVP (10 µM). After removal of the virus, conditioned supernatants were collected at 24 and 48 hours and added to naïve cells. Cell death was assessed two days later by flow cytometry. (B) THP-1 WT cells were infected or not with NL43-GFP, in the presence or absence of NVP (10 µM). Media was replaced after 6 and 24 hours. Cell death and infection were assessed by flow cytometry 48 hpi.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: (A) THP-1 WT cells were infected with NL43-GFP or not, in the presence or absence of NVP (10 µM). After removal of the virus, conditioned supernatants were collected at 24 and 48 hours and added to naïve cells. Cell death was assessed two days later by flow cytometry. (B) THP-1 WT cells were infected or not with NL43-GFP, in the presence or absence of NVP (10 µM). Media was replaced after 6 and 24 hours. Cell death and infection were assessed by flow cytometry 48 hpi.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection, Virus, Flow Cytometry

    (A-B) U937 or MonoMac6 monocytic cell lines were infected with NL43-GFP in the presence or absence of NVP (10 µM). Cell death and infection rates were analyzed by flow cytometry. (C) Primary monocytes from healthy donors were infected with NL43-GFP(+Vpx) or NL43-Luc(+Vpx). Infection was assessed by microscopy and by firefly luciferase assay, respectively, while cell death was assessed by flow cytometry. (D) THP-1 cells were infected with a minimal lentiviral vector, pLenti-GFP, in the presence or absence of NVP or RLT (10 µM). Infection and death were measured as in (A). (E) Similar to (D), except cells were infected with VSV-G pseudotyped, ROD-10 based, single-round HIV-2.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: (A-B) U937 or MonoMac6 monocytic cell lines were infected with NL43-GFP in the presence or absence of NVP (10 µM). Cell death and infection rates were analyzed by flow cytometry. (C) Primary monocytes from healthy donors were infected with NL43-GFP(+Vpx) or NL43-Luc(+Vpx). Infection was assessed by microscopy and by firefly luciferase assay, respectively, while cell death was assessed by flow cytometry. (D) THP-1 cells were infected with a minimal lentiviral vector, pLenti-GFP, in the presence or absence of NVP or RLT (10 µM). Infection and death were measured as in (A). (E) Similar to (D), except cells were infected with VSV-G pseudotyped, ROD-10 based, single-round HIV-2.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection, Flow Cytometry, Microscopy, Luciferase, Plasmid Preparation

    (A) Tet-inducible (Tet-ON) THP-1 cells were stably transduced with a codon-optimized Gag expressing vector. Cells were stimulated with doxycycline (dox; 1 µg/ml) and Gag expression was quantified by p24 staining at 24 and 48 h after treatment. (B) As in (A), except cells and supernatants were harvested, supernatants were centrifuged to pellet virus like particles, and the resulting lysates were probed for p24 expression on a Western blot. (C) THP-1 cells were infected with Tat or Rev mutant NL43-Luc viruses. Infection and death were quantified by firefly luciferase assay and live/dead staining, respectively.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: (A) Tet-inducible (Tet-ON) THP-1 cells were stably transduced with a codon-optimized Gag expressing vector. Cells were stimulated with doxycycline (dox; 1 µg/ml) and Gag expression was quantified by p24 staining at 24 and 48 h after treatment. (B) As in (A), except cells and supernatants were harvested, supernatants were centrifuged to pellet virus like particles, and the resulting lysates were probed for p24 expression on a Western blot. (C) THP-1 cells were infected with Tat or Rev mutant NL43-Luc viruses. Infection and death were quantified by firefly luciferase assay and live/dead staining, respectively.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Stable Transfection, Transduction, Expressing, Plasmid Preparation, Staining, Virus, Western Blot, Infection, Mutagenesis, Luciferase

    (A) Undifferentiated or PMA-differentiated THP-1 cells were infected with NL43-GFP (HIV-1-GFP). IL-1β and IL-18 mRNA levels were quantified by RT-qPCR two days after infection. (B) Undifferentiated THP-1 cells were infected with three-fold serial dilutions of NL4.3-GFP. mRNA levels for the indicated genes were measured by RT-qPCR two days after infection.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: (A) Undifferentiated or PMA-differentiated THP-1 cells were infected with NL43-GFP (HIV-1-GFP). IL-1β and IL-18 mRNA levels were quantified by RT-qPCR two days after infection. (B) Undifferentiated THP-1 cells were infected with three-fold serial dilutions of NL4.3-GFP. mRNA levels for the indicated genes were measured by RT-qPCR two days after infection.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection, Quantitative RT-PCR

    (A) THP-1 cells were treated with the inhibitors nevirapine (NVP; 10 µM), raltegravir (RLT; 10 µM), ruxolitinib (Ruxo; 1 µM), bafilomycin A1 (BafA1; 10 µM), chloroquine (Chlq; 10 µM), digoxin (Digo; 0.1 µM) or AZD-7648 (AZD; 10 µM) at the time of infection with NL43-GFP. Cell death was quantified two days after infection. (B) THP-1 cells were treated with inhibitors of apoptosis (Z-VAD-FMK, pan-caspase inhibitor), pyroptosis (VX765, caspase 1 inhibitor) and necroptosis (necrostatin, RIPK1 inhibitor and necrosulfonamide, MLKL inhibitor) at the indicated concentrations. Cells were challenged with NL43-GFP or mock infected, and the level of cell death was measured after two days.

    Journal: bioRxiv

    Article Title: HIV-1 infection causes depletion of monocytic cells through a non-canonical cell death pathway

    doi: 10.1101/2022.12.20.521186

    Figure Lengend Snippet: (A) THP-1 cells were treated with the inhibitors nevirapine (NVP; 10 µM), raltegravir (RLT; 10 µM), ruxolitinib (Ruxo; 1 µM), bafilomycin A1 (BafA1; 10 µM), chloroquine (Chlq; 10 µM), digoxin (Digo; 0.1 µM) or AZD-7648 (AZD; 10 µM) at the time of infection with NL43-GFP. Cell death was quantified two days after infection. (B) THP-1 cells were treated with inhibitors of apoptosis (Z-VAD-FMK, pan-caspase inhibitor), pyroptosis (VX765, caspase 1 inhibitor) and necroptosis (necrostatin, RIPK1 inhibitor and necrosulfonamide, MLKL inhibitor) at the indicated concentrations. Cells were challenged with NL43-GFP or mock infected, and the level of cell death was measured after two days.

    Article Snippet: To ensure that the observed cell death was not observed in a specific clone of THP-1 cells, we tested several THP-1 clones, as well as the reporter cell line THP-1 Lucia ISG (Invivogen) in their undifferentiated state.

    Techniques: Infection